TABLE 1

Primer sequences and PCR cycling conditions

Gene (GenBank Accession No.)	Primer Sequence (5′ to 3′)	Denaturation	Annealing	Extension
CYP3A4 (NM_017460)	CCT-TAC-ACA-TAC-ACA-CCC-TTT-GGA-AGT (forward)^{^a}	94°C, 5 s	60°C, 10 s	72°C, 15 s
	AGC-TCA-ATG-CAT-GTA-CAG-AAT-CCC-CGG-TTA (reverse)^{^a}
CYP2B6 (NM_000767)	GCG-TGT-GGT-TCA-TTC-ACA-AA (forward)^{^b}	94°C, 5 s	65°C, 5 s	72°C, 15 s
	AAT-TTA-GCC-AGG-CGT-GGT-G (reverse)^{^b}
hCAR (NM_005122)	CCA-GCT-CAT-CTG-TTC-ATC-CA (forward)^{^b}	95°C, 5 s	65°C, 10 s	72°C, 25 s
	GGT-AAC-TCC-AGG-TCG-GTC-AG (reverse)^{^b}
hPXR (NM_003889)	CAA-GCG-GAA-GAA-AAG-TGA-ACG (forward)^{^b}	95°C, 5 s	65°C, 10 s	72°C, 15 s
	CAC-AGA-TCT-TTC-CGG-ACC-TG (reverse)^{^b}
HPRT (NM_000194)	GAA-GAG-CTA-TTG-TAA-TGA-CC (forward)^{^c}	94°C, 5 s	60°C, 10 s	72°C, 15 s
	GCG-ACC-TTG-ACC-ATC-TTT-G (reverse)^{^c}