TABLE 1

Measured cellular parameters

Cellular parameters were measured during a 4-h incubation with different concentrations of CQ. pH measurements and cell volume correspond to the average over the 4-h incubation period; data correspond to the mean ± S.D. n = 4. Vesicle volume corresponds to the volume at the end of the 4-h treatment; data are presented as the mean ± S.D. n = 10.

TreatmentaVesicular pHVesicle Volume/CellVesicle Surface Area/CellCytosolic pHCell Volume (103 μm3)Extracellular pH
μm3μm2
Untreated5.03 ± 0.1521 ± 8219 ± 747.4 ± 0.21.64 ± 0.417.45 ± 0.04
25 μM CQ
    CQ/Suc5.91 ± 0.23b602 ± 1452130 ± 3497.37 ± 0.023.00 ± 0.147.48 ± 0.03
    CQ/5.36 ± 0.28304 ± 761159 ± 477.36 ± 0.041.66 ± 0.127.48 ± 0.03
    CQ/Baf5.55 ± 0.1718 ± 5189 ± 557.38 ± 0.031.74 ± 0.147.47 ± 0.03
50 μM CQ
    CQ/Suc6.08 ± 0.20843 ± 1762795 ± 4917.36 ± 0.013.05 ± 0.287.47 ± 0.03
    CQ/5.51 ± 0.34587 ± 1262207 ± 10507.36 ± 0.011.84 ± 0.057.47 ± 0.03
    CQ/Baf5.69 ± 0.3719 ± 4204 ± 247.35 ± 0.021.70 ± 0.157.47 ± 0.03
100 μM CQ
    CQ/Suc6.45 ± 0.211121 ± 3432995 ± 3857.37 ± 0.022.95 ± 0.337.50 ± 0.02
    CQ/5.81 ± 0.21294 ± 531219 ± 2347.35 ± 0.041.83 ± 0.127.50 ± 0.02
    CQ/Baf5.88 ± 0.1417 ± 3178 ± 287.34 ± 0.031.77 ± 0.147.50 ± 0.02
200 μM CQ
    CQ/Suc6.73 ± 0.28565 ± 1401468 ± 3517.37 ± 0.023.27 ± 0.377.50 ± 0.02
    CQ/5.98 ± 0.45204 ± 47785 ± 1937.30 ± 0.021.62 ± 0.177.50 ± 0.02
    CQ/Baf6.26 ± 0.4517 ± 4186 ± 327.17 ± 0.041.78 ± 0.097.50 ± 0.02

  • ↵a CQ/Suc, Suc-pretreated cells during cotreatment with CQ and sucrose; CQ/, previously untreated cells; CQ/Baf, cotreatment with CQ and Baf in previously untreated cells.

  • ↵b Values in bold indicate a statistically significant difference from the untreated cells using Tukey's test (p < 0.05).