TABLE 3

K_d and B_max for specific [³H]NMS binding in the bladder, submaxillary gland, heart, colon, lung, and brain of rats at 1 to 12 h after the oral administration of imidafenacin (6.26 μmol/kg)

Rats received 6.26 μmol/kg imidafenacin orally and were sacrificed 1 to 12 h later. The specific binding of [³H]NMS (0.06–1.5 nM) in rat tissues was measured. Values in parentheses represent the fold increase in K_d values relative to the control. Values are the mean ± S.E. for sixteen (control) and four to six (imidafenacin) rats.

Tissues and Time	K_d	B_max
	pM	fmol/mg protein
Bladder
Control	275 ± 6	146 ± 8
1 h	362 ± 13 (1.32)^**	131 ± 7
3 h	346 ± 30 (1.26)^**	140 ± 9
6 h	336 ± 21 (1.22)^*	126 ± 5
9 h	338 ± 13 (1.23)^*	143 ± 10
12 h	285 ± 23	178 ± 1
Submaxillary gland
Control	185 ± 6	116 ± 5
1 h	294 ± 19 (1.59)^**	110 ± 7
3 h	364 ± 39 (1.97)^**	114 ± 8
6 h	218 ± 5	97 ± 5
9 h	195 ± 12	122 ± 6
12 h	222 ± 9	111 ± 9
Heart
Control	423 ± 10	64.5 ± 2.3
1 h	505 ± 28 (1.19)^**	71.2 ± 3.6
3 h	500 ± 24 (1.18)^**	60.0 ± 1.1
6 h	436 ± 22	60.3 ± 2.1
9 h	462 ± 17	72.9 ± 3.5
12 h	459 ± 7	70.7 ± 3.0
Colon
Control	217 ± 7	90.3 ± 4.7
1 h	369 ± 14 (1.70)^**	74.3 ± 3.5
3 h	378 ± 46 (1.74)^**	94.3 ± 10.1
6 h	261 ± 17	97.2 ± 5.2
9 h	274 ± 39	101.7 ± 4.4
12 h	211 ± 9	70.6 ± 2.9
Lung
Control	225 ± 10	8.29 ± 0.44
1 h	325 ± 23 (1.44)^*	8.91 ± 0.72
3 h	374 ± 74 (1.66)^**	9.64 ± 0.57
6 h	244 ± 15	8.25 ± 0.82
9 h	235 ± 58	9.24 ± 1.62
12 h	264 ± 43	8.93 ± 0.86
Brain
Control	231 ± 5	1107 ± 31
1 h	257 ± 34	980 ± 17
3 h	252 ± 19	1034 ± 48
6 h	234 ± 5	975 ± 59
12 h	236 ± 7	1054 ± 69

↵* P < 0.05, significant difference from the control values.
↵** P < 0.01, significant difference from the control values.