TABLE 4

Affinities of JHW 007 determined from homologous competition assays

Values are in nM with 95% confidence limits.

RatMouse
WIN 35428 OnlyaWIN 35428 and TriprolidinebWIN 35428 OnlyaWIN 35428 and Triprolidineb
Kd 117.8 (10.0–31.6)28.3 (6.97–115)19.9 (14.7–27.0)13.2 (5.48–32.0)
Kd 212,100 (7480–19,600)11,600 (7110–19,000)9640 (6370–14,600)8750 (6410–11,900)

  • ↵a 5.6 μM unlabeled WIN 35428 (final concentration) in all tubes.

  • ↵b 5.6 μM unlabeled WIN 35428 and 28 μM unlabeled triprolidine (final concentrations) in all tubes.