TABLE 3 1 mM glycine, but not 1 mM glutamate, ability to reverse selected HTS hits inhibitory effect in NR1/NR2A FLIPR/Ca2+ assays
Values are pIC50 mean ± S.D. from NR1/NR2A FLIPR/Ca2+ assay in the presence of 1 mM glycine (Gly) or 1 mM L-glutamate (Glu), added 5 min after compound addition. The number of independent experiments is indicated in parentheses. Maximal inhibition was 92 to 100% for all listed compounds. The ability of 1 mM glycine to surmount inhibitory effect of compounds 1–3, 6, and 13 is reflected in pIC50 values <4.3, measured 5 min after glycine addition. Glycine (1 mM) is not able to surmount NVP-AAM077 inhibitory effect. (+)-MK-801 inhibitory effect was not surmounted by either of the two agonists.