Metabolism of nifedipine in primary cultures of human hepatocytes
Metabolism of nifedipine was quantified by comparing the ratio of peak area of oxidized nifedipine to that of nitrendipine in HPLC analyses. The data are indicated as induction folds of the production of oxidized nifedipine (CYP3A4 metabolite) compared with the DMSO-treated group in each donor.
| Donor | Drug Treatment | |||||
|---|---|---|---|---|---|---|
| DMSO | CPT (1 μM) | Iri (1 μM) | Rif (10 μM) | Rif (10 μM) + CPT (1 μM) | Rif (10 μM) + Iri (1 μM) | |
| 1 | 1 | —a | — | 7.6 | 3.9 | — |
| 2 | 1 | 2 | 1.6 | 26 | 2 | 21 |
| 3 | 1 | 1.5 | — | 17 | 12 | — |
Iri, irinotecan; Rif, rifampicin.
↵a —, not determined.