TABLE 1

Pharmacological characterization of the different CHO cell lines stably expressing the human β1–3 adrenoceptors

Levels of receptor expression (expressed in pmol/mg of protein content in membrane preparations) were determined in saturation binding assays, as described under Materials and Methods. The average of at least three independent experiments performed in triplicate is shown.

Cell LineBmaxUse
pmol/mg
CHO-hβ1-HIGH12.4 ± 0.3Binding experiments
CHO-hβ2-HIGH1.6 ± 0.1
CHO-hβ3-HIGH22.0 ± 1.6
CHO-hβ1-LOW0.52 ± 0.04Functional experiments (cAMP)
CHO-hβ2-LOW0.056 ± 0.009
CHO-hβ3-LOW0.23 ± 0.06