Comparisons of proteins that were altered in iTRAQ experiments to tox lists in the Ingenuity Knowledge Base
Data sets were examined by IPA software for overlap with tox list proteins. Tox lists are lists of molecules that are known to be involved in particular aspects of toxicity, and our focus was on lists that addressed nuclear receptor activation and xenobiotic metabolism. Underlined entries were not common to the datasets of both experiments.
| Tox List ID (Total number of list members) | STZ | STZ + Insulin | ||
|---|---|---|---|---|
| Up-Regulated | Down-Regulated | Up-Regulated | Down-Regulated | |
| CAR/RXR activation (28) | UGT1A1 | — | — | CYP2B6 |
| FXR/RXR activation (66) | ABCB4, APOA1, G6PC, SLC27A5 | FASN | — | — |
| LXR/RXR activation (55) | APOA1 | ACACA, CD36, FASN | LDLR | CD36 |
| Nrf2 activation (148) | CYP2A1, CYP4A11, CYP4A14 | HMOX1 | GSTA4, GSTM1, HSP90AA1 | — |
| PPARα/RXR activation (118) | ACADL, APOA1 | CD36, FASN | HSP90AA1 | CD36 |
| PXR/RXR activation (57) | ALDH1A7, G6PC, HMGCS2, UGT1A1 | — | GSTM1 | CYP2B6 |
| Cytochrome P450 panel (rat)–substrate is a xenobiotic (23) | CYP2A1, CYP2D3 | — | CYP2D3 | CYP2B6 |
| Xenobiotic metabolism (89) | CYP2A1, CYP2B9, CYP2D3, CYP51A1, UGT1A1 | — | ALDH1L1, CYP2D3, CYP4F2, GSTA4, GSTM1 | CYP2B6, UGT1A4 |