Conditions for receptor binding assays
| Receptor | Preparation | [3H] Ligand | Displacer | Incubation Temperature and Time | References |
|---|---|---|---|---|---|
| (nM) | (μM) | ||||
| Dopamine D2 | Rat striatum | [3H]spiperone (0.5) | Spiperone (10) | 37°C, 10 min | Hirose et al., 1990 |
| 5-HT1A | Rat hippocampus | [3H]8-OH-DPAT (0.15) | 8-OH-DPAT (1) | 25°C, 30 min | Kato et al., 1990 |
| 5-HT2A | Rat cortex | [3H]ketanserin (1) | Ketanserin (10) | 37°C, 30 min | Hirose et al., 1990 |
| 5-HT7 | Human recombinant | [3H]5-CT (1) | 5-HT (1) | r.t., 2 h | To et al., 1995 |
| Noradrenaline α1 | Rat cortex | [3H]prazosin (0.5) | Prazosin (1) | 25°C, 30 min | Kato et al., 1990 |
| Noradrenaline α2A | Human recombinant | [3H]MK-912 (0.7) | WB4101 (10) | 27°C, 60 min | Uhlén et al., 1998 |
| Noradrenaline α2C | Human recombinant | [3H]MK-912 (0.2) | WB4101 (10) | 27°C, 60 min | Uhlén et al., 1998 |
| Histamine H1 | Guinea pig whole brain | [3H]pyrilamine (0.4) | Triprolidine (2) | 25°C, 40 min | Chang et al., 1979 |
| Muscarinic | Rat cortex | [3H]QNB (0.15) | Oxotremorine (100) | 25°C, 60 min | Yamamura et al., 1974 |
r.t., room temperature; 5-CT, 5-carboxamidotryptamine; MK-912, (2S,12bS)-1′,3′-dimethylspiro[1,3,4,5′,6,6′,7,12b-octahydro-2H-benzo[b]furo[2,3-a]quinolizine-2,4′-pyrimidin]-2′-one; QNB, quinuclidinyl benzilate.