TABLE 3

PF-3644022 inhibition of LPS-induced MK2 activity and cytokine production in human cells and whole blood

Data are expressed as mean values ± S.E.M. for multiple independent experiments as follows: U937 TNFα, n = 8; U937 MK2 activity, n = 5; hPBMC cytokines, n = 4; HWB MK2 activity, n = 3; and HWB cytokines, n = 6. For hPBMC and HWB studies, each independent experiment represents an individual blood donor.

AssayIC50
μM
U937-TNFα0.159 ± 0.014
U937 MK2 activity0.201 ± 0.042
hPBMC-TNFα0.160 ± 0.069
hPBMC-IL-1β2.78 ± 0.51
hPBMC-IL-61.26 ± 0.17
hPBMC-IL-82.09 ± 0.51
HWB-TNFα1.97 ± 0.80
HWB MK2 activity1.60 ± 0.53
HWB-IL-1β> 25
HWB-IL-610.3 ± 2.33
HWB-IL-8> 25