Effects of TRO40303 and CsA on mPTP opening after hypoxia/reoxygenation
Cardiomyocytes were coloaded with calcein and CoCl2, and mPTP opening was induced by in vitro hypoxia/reoxygenation. The percentage of cells undergoing rapid or gradual opening and average time to mPTP opening for the cell population in which mPTP opened after reoxygenation was calculated. Statistical analysis was performed by one-way ANOVA versus control followed by Dunnett's post-test.
| Cells with Rapid mPTP Opening | Cells with Gradual mPTP Opening | Average TmPTP50 | |
|---|---|---|---|
| % | Min | ||
| Control (n = 5) | 95.2 ± 2.8 | 4.8 ± 2.8 | 34.5 ± 4.3 |
| 1 μM CsA (n = 3) | 35.4 ± 13.1** | 64.6 ± 13.1** | 67.9 ± 3.7*** |
| 3 μM TRO40303 (n = 3) | 34.3 ± 6.3** | 65.7 ± 6.3** | 69.3 ± 9.4*** |