Respiratory activities and membrane potential in mitochondria isolated from rats fed the CTRL diet, the MCD diet, and the MCD plus SILIPHOS diet at 7 and 14 weeks
Glutamate-malate or succinate was used as the oxidative substrate. Data are expressed as means ± S.D.M. of five experiments for each group. Statistical differences were assessed using ANOVA and the Tukey-Kramer multicomparison test.
| CTRL | MCD | SILIPHOS | |
|---|---|---|---|
| Glutamate-malate | |||
| State 3 (nmol O2/min/mg protein) | |||
| 7th Week | 88.67 ± 12.28 | 59.28 ± 9.45* | 77.94 ± 10.06** |
| 14th Week | 90.47 ± 13.97 | 51.73 ± 11.47*** | 74.53 ± 9.61** |
| State 4 (nmol O2/min/mg protein) | |||
| 7th Week | 26.15 ± 5.01 | 18.02 ± 3.68* | 20.1 ± 4.6 |
| 14th Week | 25.07 ± 5.32 | 15.83 ± 5.83* | 20.01 ± 5.46 |
| Respiratory control index | |||
| 7th Week | 4.39 ± 0.34 | 3.12 ± 0.37* | 3.98 ± 1.13 |
| 14th Week | 3.71 ± 0.44 | 3.01 ± 0.19* | 3.76 ± 0.83 |
| Membrane potential (mV) | |||
| 7th Week | 175.45 ± 8.11 | 152.06 ± 4.51* | 171.15 ± 6.99** |
| 14th Week | 179.41 ± 7.55 | 149.22 ± 6.54* | 173.76 ± 4.98* |
| Succinate | |||
| State 3 (nmol O2/min/mg protein) | |||
| 7th Week | 172.68 ± 27.54 | 148.2 ± 24.76 | 145.64 ± 22.46 |
| 14th Week | 168.92 ± 28.27 | 142.76 ± 20.80 | 143.50 ± 21.27 |
| State 4 (nmol O2/min/mg protein) | |||
| 7th Week | 58.3 ± 12.58 | 42.7 ± 15.95 | 47.31 ± 20.39 |
| 14th Week | 55.37 ± 13.10 | 49.96 ± 6.1 | 51.65 ± 17.41 |
| Respiratory control index | |||
| 7th Week | 3.12 ± 0.39 | 3.46 ± 1.17 | 3.29 ± 0.47 |
| 14th Week | 3.06 ± 0.84 | 2.93 ± 0.69 | 2.99 ± 0.49 |
| Membrane potential (mV) | |||
| 7th Week | 193.62 ± 5.91 | 170.26 ± 3.90* | 201.07 ± 5.86** |
| 14th Week | 189.56 ± 2.91 | 159.08 ± 10.40* | 168.27 ± 5.73* |