Primer sequence for RT-PCR
| Receptor | Primer (5′–3′) | Nucleotide Position | Transcript Size |
|---|---|---|---|
| bp | |||
| D1R (GenBank Accession number NM_000794) | F: CCTGTTTCCTATCGCTGCTC | 80–99 | 491 |
| R: TGAGGCTGGAGTCACAGTTG | 552–571 | ||
| D5R (GenBank Accession number NM_000798) | F: GACGGTGAACATCAGCAATG | 1551–1570 | 212 |
| R: CCCAGACAGACTCAGCAACA | 1743–1762 |
F, forward; R, reverse.