Effects of cucurbitacin R on TNF-α, IL-1β, and IL-4 in DTH induced by DNFB
| DTH Induced by DNFB | ||||||
|---|---|---|---|---|---|---|
| TNF-α | IL-1β | IL-4 | ||||
| pg/ml ± S.E.M. | %I | pg/ml ± S.E.M. | %I | pg/ml ± S.E.M. | %I | |
| Blank | n.d. | n.d. | 16 ± 1 | |||
| Control | 114 ± 19 | 99 ± 21 | 25 ± 3 | |||
| Cucurbitacin R | 97 ± 28* | 15 | 65 ± 12 | 34 | 14 ± 1* | 128 |
| Dexamethasone | n.d.* | 100 | n.d.* | 100 | 18 ± 3* | 87 |
↵ Percentages of inhibition were calculated from values of test compounds minus blank (protein extract of animals treated with vehicle) versus control (protein extract of animals treated with DTH reagent) minus blank. Values are expressed as picograms of each cytokine production per milliliter ± S.E.M.; n.d., no detected amounts. Statistically significant difference with respect to the control is expressed as * P < 0.01 (Dunnett's t test).