Selective ligands enhance PPAR-γ protein expression in monocytes and MDM
Monocytes and MDM from five different donors per group were stimulated (6 h at 37°C in 5% CO2) with the natural ligand 15d-PGJ2 (10 μM) or the synthetic agonist Rgtz (5 μM). PPAR-γ/β -actin ratio was measured as described in text, and results are expressed as -fold increase over control, unstimulated cells. Values are mean ± S.E.M. p value, not significant between cell types and donors.
| -Fold Increase in PPAR-γ/β-Actin Ratio | ||
|---|---|---|
| + 10 μM 15d-PGJ2 (n = 5) | + 5 μM Rgtz (n = 5) | |
| Monocytes, healthy, M | 2.5 ± 0.3 | 4 ± 0.4 |
| Monocytes, healthy, F | 2.6 ± 0.4 | 3.9 ± 0.3 |
| Monocytes, CAD, M | 2.7 ± 0.3 | 4 ± 0.3 |
| Monocytes, CAD, F | 2.8 ± 0.3 | 4.2 ± 0.4 |
| MDM, healthy, M | 2.6 ± 0.2 | 4 ± 0.3 |
| MDM, healthy, F | 2.7 ± 0.3 | 4.2 ± 0.4 |
| MDM, CAD, M | 2.7 ± 0.2 | 4.1 ± 0.4 |
| MDM, CAD, F | 2.8 ± 0.4 | 4.4 ± 0.3 |
CAD, patients with coronary artery disease; F, females; M, males.