Table 3

Summary of cAMP assay data

Data are mean pA2 ± s.e.m. of three to five independent experiments performed in triplicate.

Mean pA2 ± s.e.m. (n)Log Fold Selectivity, AM1 vs. AM2
AM1AM2CGRP
AM22–527.40 ± 0.14 (3)6.86 ± 0.09 (3)5.96 ± 0.09 (3)+0.54
AM26–526.34 ± 0.17 (3)5.92 ± 0.04 (3)<5 (4)+0.42
AM30–526.03 ± 0.35 (3)6.04 ± 0.16 (3)<5 (4)−0.01
C17.41 ± 0.11 (3)6.78 ± 0.21 (3)6.97 ± 0.17 (4)+0.63
C28.39 ± 0.16 (4)8.53 ± 0.17 (4)8.25 ± 0.10 (4)−0.14
C36.00 ± 0.36 (3)5.94 ± 0.10 (3)5.87 ± 0.13 (3)+0.06
C46.16 ± 0.07 (3)5.66 ± 0.24 (3)5.76 ± 0.01 (4)+0.5
C5<5 (3)<5 (5)5.49 ± 0.06 (3)
C68.37 ± 0.18 (3)8.10 ± 0.08 (4)8.21 ± 0.10 (3)+0.27
C77.25 ± 0.13 (3)7.81 ± 0.20 (4)6.29 ± 0.19 (3)−0.56
C86.84 ± 0.14 (3)6.90 ± 0.15 (3)6.36 ± 0.10 (3)−0.06
C96.85 ± 0.21 (3)7.02 ± 0.21 (3)6.91 ± 0.06 (3)a−0.17
  • a C9 affinity was determined in COS-7 cells transfected with CGRP receptors, whereas the other antagonists were tested in SK-N-MCs, which endogenously express this receptor. AM22–52 affinity was not significantly different between these cells [SK-N-MC pA2 = 5.96 ± 0.09 (n = 3) vs. COS-7 pA2 = 5.54 ± 0.16 (n = 4); not significantly different by t test].