Measurement of 3-HPMA in urine of WT and GSTP-null mice
HPMA calculated as total micrograms in urine volume collected over 15 h pre- and post-treatment with CY (50 mg/kg, i.p., n = 5, 5 mice, respectively) or acrolein (2 mg/kg, i.p.; n = 4, 5 mice, respectively). Values are mean ± S.E.M.
| WT (n = 5, 4) | GSTP-Null (n = 5, 5) | |
|---|---|---|
| Pre-CY treatment | 4.13 ± 0.26 | 4.06 ± 0.20 |
| Post-CY treatment | 22.06 ± 1.66 | 18.11 ± 1.9 |
| Ratio (Post/Pre: CY) | 5.42 ± 0.49 | 4.46 ± 0.42 |
| Pre-acrolein treatment | 4.07 ± 0.44 | 3.16 ± 0.42 |
| Post-acrolein treatment | 21.41 ± 1.93 | 24.34 ± 2.37 |
| Ratio (Post/Pre: acrolein) | 5.38 ± 0.60 | 7.93 ± 0.58* |
↵* p < 0.05 between WT and GSTP-null mice.