Cellular activity of BMS-066 against LPS-induced responses in human peripheral blood mononuclear cells
| Endpoint | Detection | IC50 |
|---|---|---|
| μM | ||
| TNF-α (protein) | Immunoassay | 0.27 ± 0.17 |
| IκBα phosphorylation | Immunoassay | 0.29 ± 0.10 |
| TNF-α (mRNA) | qPCR | 0.18 ± 0.06 |
| IL-6 (mRNA) | qPCR | 0.16 ± 0.05 |
| IL-12/p40 (mRNA) | qPCR | 0.12 ± 0.04 |
| IL-23/p19 (mRNA) | qPCR | 0.19 ± 0.06 |
qPCR, quantitative real-time polymerase chain reaction.