Primers used to determine Gαi isoform expression by quantitative reverse transcription-PCR
Gene | Primer Sequence [5′ → 3′] | GenBank Accession No. | Product Size | |
---|---|---|---|---|
bp | ||||
Gαi1 | ACGATTCGGCAGCGTACT | s | NM_010305 | 72 |
TCCTGCTGAGTTGGGATGTA | as | |||
Gαi2 | GAGGTGAAGTTGCTTCTGTTAGG | s | NM_008138 | 77 |
TTCATGGATGATCTTCATCTGC | as | |||
Gαi3 | GATTGATTTTGGGGAATCTGC | s | NM_010306 | 85 |
AATCACGCCTGCTAGTTCTGA | as | |||
Rps29 | ATGGGTCACCAGCAGCTCTA | s | NM_009093 | 154 |
AGCCTATGTCCTTCGCGTACT | as |
bp, base pair; Rps29, ribosomal protein S29; s, sense; as, antisense.