TABLE 1

Oligonucleotide sequences for site-directed mutagenesis of the human PXR protein

Amino AcidOligos for Mutagenesis to AOligos for Mutagenesis to D
Ser8 5′-ggaggtgagacccaaagaagcctggaaccatgctg-3′ 5′-ggaggtgagacccaaagaagactggaaccatgctg-3′
3′-cagcatggttccaggcttctttgggtctcacctcc-5′ 3′-cagcatggttccagtcttctttgggtctcacctcc-5′
Thr20 5′-tgtacactgtgaggacgcagagtctgttcctgg-3′ 5′-gactttgtacactgtgaggacgatgagtctgttcctggaaagccc-3′
3′-ccaggaacagactctgcgtcctcacagtgtaca-5′ 3′-gggctttccaggaacagactcatcgtcctcacagtgtacaaagtc-5′
Thr57 5′-ctatcacttcaatgtcatggcatgtgaaggatgcaaggg-3′ 5′-ctggctatcacttcaatgtcatggattgtgaaggatgcaagggcttttt-3′
3′-cccttgcatccttcacatgccatgacattgaagtgatag-5′ 3′-aaaaagcccttgcatccttcacaatccatgacattgaagtgatagccag-5′
Thr90 5′-gatcacccggaaggcccggcgacagtg-3′ 5′-agatcacccggaaggaccggcgacagtgcc-3′
3′-cactgtcgccgggccttccgggtgatc-5′ 3′-ggcactgtcgccggtccttccgggtgatct-5′
Ser105 5′-cgcaagtgcctggaggccggcatgaagaagga-3′ 5′-gcgcaagtgcctggaggacggcatgaagaaggag-3′
3′-tccttcttcatgccggcctccaggcacttgcg-5′ 3′-ctccttcttcatgccgtcctccaggcacttgcgc-5′
Ser114 5′-gaaggagatgatcatggccgacgaggccgtg-3′ 5′-gaaggagatgatcatggacgacgaggccgtggag-3′
3′-cacggcctcgtcggccatgatcatctccttc 3′-ctccacggcctcgtcgtccatgatcatctccttc-5′
Ser130 5′-cttgatcaagcggaagaaagctgaacggacagggactcagc-3′ 5′-cttgatcaagcggaagaaagacgaacggacagggactcagc-3′
3′-gctgagtccctgtccgttcagctttcttccgcttgatcaag-5′ 3′-gctgagtccctgtccgttcgtctttcttccgcttgatcaag-5′
Thr133 5′-gaagaaaagtgaacgggcagggactcagccact-3′ 5′-cggaagaaaagtgaacgggatgggactcagccactggga-3′
3′-agtggctgagtccctgcccgttcacttttcttc-5′ 3′-tcccagtggctgagtcccatcccgttcacttttcttccg-5′
Thr135 5′-tgaacggacaggggctcagccactggg-3′ 5′-aagtgaacggacaggggatcagccactgggagtg-3′
3′-cccagtggctgagcccctgtccgttca-5′ 3′-cactcccagtggctgatcccctgtccgttcactt-5′
Ser180 5′-caggggtgcttagcgctggctgcgagttgc-3′ 5′-ccaggggtgcttagcgatggctgcgagttgcc-3′
3′-gcaactcgcagccagcgctaagcacccctg-5′ 3′-ggcaactcgcagccatcgctaagcacccctgg-5′
Ser192 5′-ctctgcaggccccagcgagggaagaag-3′ 5′-cagagtctctgcaggccccagatagggaagaagctgcc-3′
3′-cttcttccctcgctggggcctgcagag-5′ 3′-ggcagcttcttccctatctggggcctgcagagactctg-5′
Ser208 5′-tccggaaagatctgtgcgctttgaaggtctctctg-3′ 5′-ggtccggaaagatctgtgcgatttgaaggtctctctgcag-3′
3′-cagagagaccttcaaagcgcacagatctttccgga-5′ 3′-ctgcagagagaccttcaaatcgcacagatctttccggacc-5′
Ser231 5′-acccccagccgacgctggcgggaaagag-3′ 5′-caaacccccagccgacgatggcgggaaagagatc-3′
3′-ctctttcccgccagcgtcggctgggggt-5′ 3′-gatctctttcccgccatcgtcggctgggggtttg-5′
Ser274 5′-cgaggaccagatcgccctgctgaaggg-3′ 5′-atcgaggaccagatcgacctgctgaagggggc-3′
3′-cccttcagcagggcgatctggtcctcg-5′ 3′-gcccccttcagcaggtcgatctggtcctcgat-5′
Thr290 5′-gtcaactgagattcaacgcagtgttcaacgcggag-3′ 5′-ctgtgtcaactgagattcaacgatgtgttcaacgcggagactgga-3′
3′-ctccgcgttgaacactgcgttgaatctcagttgac-5′ 3′-tccagtctccgcgttgaacacatcgttgaatctcagttgacacag-5′
Ser305 5′-gtgtggccggctggcctactgcttgga-3′ 5′-ggagtgtggccggctggactactgcttggaagac-3′
3′-tccaagcagtaggccagccggccacac-5′ 3′-gtcttccaagcagtagtccagccggccacactcc-5′
Ser350 5′-catctccctcttcgccccagaccgccc-3′ 5′-ccatctccctcttcgacccagaccgcccag-3′
3′-gggcggtctggggcgaagagggagatg-5′ 3′-ctgggcggtctgggtcgaagagggagatgg-5′
Thr408 5′-caatgctcagcacgcccagcggctgct-3′ 5′-atcaatgctcagcacgaccagcggctgctgcg-3′
3′-agcagccgctgggcgtgctgagcattg-5′ 3′-cgcagcagccgctggtcgtgctgagcattgat-5′

  • Oligos, oligonucleotide sequences.