Peptidase inhibitors and amino acids that did not substantially affect the binding characteristics of 125I-Ang II to rat hypothalamic membrane AT1 receptors when added to the initial assay buffer
| Peptidase Inhibitor/Amino Acid | Concentration |
|---|---|
| μM | |
| Amastatin | 3–30 |
| Bestatin | 10–100 |
| Leuhistin | 2 and 5 |
| Pepstatin A | 10 |
| Plummer's reagent | 100 |
| Phosphoramidon | 10 |
| 2-Pro-Pro-OH | 100–1000 |
| Ile-Pro-OH | 1000 and 3000 |
| Glu-SH | 40–200 ± 1 mM dithiothreitol |
| JA-2 | 10 |
| ZnCl2 | 500–5000 |
| Ala | 1000 |
| Arg | 1000 |
| Asn | 1000 |
| Glu | 1000 |
| Ile | 1000 |
| Phe | 1000 |