Metabolism of 125I-Ang II and 125I-SI Ang II bound to rat hypothalamic and liver membrane preparations in assay buffer with additional protease inhibitors (Materials and Methods)
Data are mean ± S.E.M., n = 3.
| Tyr | Ang (1–4) or S-Ang (1–4) | Ang (4–7) | Ang (1–7) | Ang (2–7) | Ang (4–8) | Ang III | Ang II or SI Ang II | |
|---|---|---|---|---|---|---|---|---|
| % | ||||||||
| Hypothalamic membranes | ||||||||
| 125I-Ang II | 1.37 ± 0.19 | 2.8 ± 0.75 | 4.1 ± 1.6 | 4.49 ± 1.1 | 10.8 ± 1.3 | 75.6 ± 3.2 | ||
| 125I-SI Ang II | 4.56 ± 0.1 | 7.2 ± 2.9 | 2.7 ± 0.6 | 78.8 ± 1.1 | ||||
| Liver membranes | ||||||||
| 125I-Ang II | 0.83 ± 0.18 | 95.3 ± 1.7 | ||||||
| 125I-SI Ang II | 0.64 ± 0.1 | 0.45 ± 0.03 | 1.7 ± 2.5 | 95.3 ± 0.5 | ||||