Results of Schild analyses of antagonism of midazolam substitution for the discriminative stimulus effects of 1.0 and 2.0 g/kg ethanol by Ro15-4513 or flumazenil in cynomolgus monkeys
Apparent pA2 for individual monkeys was calculated when three or more shifts in the dose-response function (n) were obtained and the Schild slope was between −0.4 and −1.6. Apparent pKB was calculated when less than three shifts in the dose-response function (n) were obtained.
| Females | Males | ||||||
|---|---|---|---|---|---|---|---|
| n | Slope | pA2a (pKB) | n | Slope | pA2 (pKB) | ||
| Ro15-4513 (1.0 g/kg ethanol training) | |||||||
| 3123 | 3 | −1.02 | 7.23 | 4889 | 3 | −1.89 | NC |
| 2830 | 4 | −1.07 | 7.19 | 4892 | 2 | NC | (7.57) |
| 2852 | 3 | −0.60 | 7.19 | 4865 | 0 | ||
| 2913 | 3 | −0.95 | 7.15 | ||||
| Mean | −0.91 | 7.19 | Mean | −1.89 | NC (7.57) | ||
| 95% CI | −0.50, −1.32 | 7.13, 7.26 | 95% CI | NC | NC (7.47, 7.66) | ||
| Ro15-4513 (2.0 g/kg ethanol training) | |||||||
| 3220 | 3 | −0.36 | NC | 4890 | 3 | −0.08 | NC |
| 5400 | 2 | NC | (6.67) | ||||
| 2835 | 2 | NC | (7.85) | ||||
| 5999 | 3 | −0.87 | 6.58 | ||||
| Mean | −0.62 | 6.58 (7.26) | Mean | −0.08 | NC | ||
| 95% CI | 0.09, −1.32 | NC (5.89, 8.64) | 95% CI | NC | NC | ||
| Flumazenil (1.0 g/kg ethanol training) | |||||||
| 3123 | 0 | 4892 | 3 | NC | (7.22) | ||
| 2830 | 1 | NC | (7.36) | 4889 | 2 | NC | (6.13) |
| 2852 | 3 | −0.44 | 7.18 | ||||
| Mean | −0.44 | 7.18 (7.36) | Mean | NC | (6.68) | ||
| 95% CI | NC | NC | 95% CI | NC | (5.42, 7.94) | ||
| Flumazenil (2.0 g/kg ethanol training) | |||||||
| 3220 | 5 | −0.74 | 6.34 | 4890 | 3 | −1.31 | 7.09 |
| 5999 | 3 | −1.81 | NC | ||||
| Mean | −1.27 | 6.34 | Mean | −1.31 | 7.09 | ||
| 95% CI | −0.21, −2.76 | NC | 95% CI | NC | NC | ||
↵a For all pA2 analyses slopes were constrained to −1; NC, not calculable.