The enzyme inhibitors, URB597 and JZL184, selectively increased brain and spinal cord levels of AEA and 2-AG, respectively Fourteen days after CCI/sham surgery, mice were injected with URB597 (10 mg/kg i.p.), JZL184 (16 mg/kg i.p.), or vehicle. Pretreatment times were 1 h for URB597 and 2 h for JZL184, along with each vehicle. LC/MS/MS was used to quantify AEA and 2-AG for each sample. Data are expressed as mean (± S.E.M.). n = 4 to 6.
Brain | Spine | ||||||
|---|---|---|---|---|---|---|---|
| AEA | 2-AG | AEA | 2-AG | ||||
| pmol/g | nmol/g | pmol/g | nmol/g | ||||
| VEH | CCI | 3.76 (0.51) | 14.55 (1.95) | 7.19 (3.71) | 14.77 (3.46) | ||
| Sham | 3.30 (0.36) | 14.95 (1.91) | 5.21 (2.18) | 15.82 (3.82) | |||
| URB597 | CCI | 36.30 (9.80)** | 13.18 (2.41) | 180.44 (89.51) | 12.68 (2.90) | ||
| Sham | 30.21 (9.37)** | 12.05 (1.91) | 115.15 (62.42) | 10.79 (1.48) | |||
| JZL184 | CCI | 5.90 (1.23) | 85.83 (17.75)*** | 14.07 (2.50) | 76.32 (15.58)** | ||
|
| Sham | 5.35 (1.68) | 132.16 (36.64)** | 12.79 (5.41) | 44.64 (15.53) | ||