TABLE 1

Primers used for real-time PCR amplification

Gene
GenBank Accession No.
Forward Primer (5′–3′), Reverse Primer (5′–3′)
PCR Product Size
bp
Bcl-2 NM_016993.1 GGGATGCCTTTGTGGAACT, CTGAGCAGCGTCTTCAGAGA 82
Caspase-3 NM_012922.2 AAACCTCCGTGGATTCAAAA, AGCCCATTTCAGGGTAATCC 123
eNOS NM_021838.2 TGGAAATTAACGTGGCTGTG, GCCTTCTGCTCATTTTCCAA 112
MMP-9 NM_031055.1 AAAAGGCATCCAGCATCTGT, AGCTGTCGGCTGTGGTTC 88
TNF-α BC107671.1 GTCTACTGAACTTCGGGGTGA, ATGAGAGGGAGCCCATTTG 67
β-Actin
NM_031144.2
CTAAGGCCAACCGTGAAAAG, TACATGGCTGGGGTGTTGA
64

  • bp, base pairs