In vitro saturation binding of [N-methyl-3H3]AZ10419369 at the 5HT1B receptor Affinity and receptor density was determined by using [N-methyl-3H3]AZ10419369 in membranes from cells expressing human (transfected CHO cell) or guinea pig (transfected HEK cell) 5-HT1B receptors or from monkey (cortex) and guinea pig (cortex, striatum, and substantia nigra) brain tissue.
| [N-methyl-3H3]AZ10419369 | Human Transfected CHO Cell | Guinea Pig Transfected HEK Cell | Monkey Cortex | Guinea Pig | ||
|---|---|---|---|---|---|---|
| Cortex | Striatum | Substantia Nigra | ||||
| Kd (nM) | 0.37 | 0.38 | 3.85 | 8.1 | 1.9 | 1.5 |
| Bmax (pmol/mg protein) | 8.76 | 13.013 | 0.131 | 0.349 | 0.099 | 0.115 |