Equilibrium binding of [3H]SAG-1.3 and [3H]cyclopamine to human smoothened receptors in HEK293F cell membranes Kd affinity and Bmax receptor density were determined using nonlinear curve fitting as described under Data Analysis in text. Data are the mean ± S.D. (n = 2–7).
Radioligand | Method | Kd | Bmax |
|---|---|---|---|
| nM | pmol / mg protein | ||
| [3H]SAG-1.3 | Radioactive saturation | 1.7 ± 0.5 | 34 ± 2.3 |
| [3H]SAG-1.3 | Isotopic unlabeled | 5.9 ± 1.7 | 40 ± 8.0 |
| [3H]Cyclopamine | Isotopic unlabeled | 10 ± 2.5 | 20 ± 0.9 |