Histological analysis of joint tissue from vehicle- and HE3286-treated animals Two hind limbs from three to four mice were submitted in paraformaldehyde. Limbs were decalcified in HCl and sectioned in half longitudinally through the stifle and tibiotarsal joints. Five-micrometer sections were processed and stained with hematoxylin and eosin.
Dose | Stiffle Joint | Tibiotarsal Joint and Tarsal Joints | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| SPS | AIS | Notes | SPS | AIS | Notes | |||||
| 40 mg/kg | 0 | 0 | Very few mononuclear cells | 2 | 3 | Tibiotarsal joint was unremarkable. Tarsal joints had moderate synovial proliferation into articular spaces. Multifocal neutrophilic and mononuclear inflammatory cells are present within the synovium and adjacent stroma. | ||||
| 40 mg/kg | 0 | 0 | Very few mononuclear cells | 1 | 1 | Mild synovial proliferation is present and scattered mononuclear cells in stroma. | ||||
| 0 mg/kg | 3 | 2 | Marked synovial proliferation and growth into adjacent bone and cartilage. Erosion and loss of articular cartilage are present. Scattered mononuclear and neutrophilic inflammatory cells are present within the synovium and adjacent stroma. | 3 | 3 | Marked synovial proliferation and in growth into adjacent bone and cartilage, and filling in the articular spaces. Marked erosion and loss of articular cartilage are present. Multifocal neutrophilic and mononuclear inflammatory cells are present within the synovium and adjacent stroma. | ||||
| 0 mg/kg | 2 | 1 | Moderate synovial proliferation is present and scattered mononuclear cells in stroma and synovial space contains a focal area of neutrophils. | 0 | 0 | Section through tibiotarsal joint was incomplete. However. tarsal joints were overall unremarkable. | ||||
| 0 mg/kg | 3 | 2 | Marked synovial proliferation and growth into adjacent bone and cartilage. Erosion and loss of articular cartilage are present. Scattered mononuclear and neutrophilic inflammatory cells are present within the synovium and adjacent stroma. | 3 | 3 | Marked synovial proliferation and in growth into adjacent bone and cartilage, and filling in the articular spaces. Marked erosion and loss of articular cartilage are present. Multifocal neutrophilic and mononuclear inflammatory cells are present within the synovium and adjacent stroma with associated necrosis. | ||||
| 0 mg/kg | 3 | 2 | Marked synovial proliferation and growth into adjacent bone and cartilage. Erosion and loss of articular cartilage are present. Scattered mononuclear and neutrophilic inflammatory cells are present within the synovium and adjacent stroma. | 3 | 3 | Marked synovial proliferation and in growth into adjacent bone and cartilage, and filling in the articular spaces. Marked erosion and loss of articular cartilage are present. Multifocal neutrophilic and mononuclear inflammatory cells are present within the synovium and adjacent stroma with associated necrosis. | ||||
| 40 mg/kg | N.D. | N.D. | Processing artifact prevented adequate evaluation. | N.D. | N.D. | Processing artifact prevented adequate evaluation. | ||||
| 40 mg/kg | 3 | 3 | Marked synovial proliferation and in growth into adjacent bone and cartilage. Erosion and loss of articular cartilage are present. Diffuse mononuclear and neutrophilic inflammatory cells are present within the synovium and adjacent stroma. The articular spaces contain large numbers of neutrophils and fibrin. | 0 | 0 | Section through tibiotarsal joint was incomplete. However, tarsal joints were overall unremarkable. | ||||
N.D., not done