Effect of late treatment with methylene blue (20 h after the CLP procedure) on several variables
Rats were injected with PBS or MB (10 mg/kg s.c.) 20 h after CLP. Thirty-six hours after surgery, the rats were instrumented for heart rate measurement followed by blood and tissue harvesting as described in Table 1. Variables are as in Table 1. Data are expressed as mean ± S.E.M. of six to eight animals per group. Statistical analysis was performed using two-way ANOVA followed by Bonferroni's post hoc t test.
Parameter | Groups | ||||||
|---|---|---|---|---|---|---|---|
| Sham/PBS | Sham/MB | CLP/PBS | CLP/MB | ||||
| Isoprenaline response | 148.7 ± 14.4 | 152.2 ± 12.6 | 84.5 ± 15.1* | 77.6 ± 18.1* | |||
| Leukocytes | 14.2 ± 3.2 | 15.3 ± 4.1 | 6.2 ± 2.7* | 5.8 ± 3.0* | |||
| MPO activity | 41.4 ± 5.1 | 39.4 ± 3.4 | 89.00.1 ± 2.8* | 103.1 ± 9.3* | |||
| Lactate | 268.6 ± 15.7 | 282.7 ± 31.2 | 490.1 ± 68.2* | 274.1 ± 45.8# | |||
| Urea | 17.6 ± 1.8 | 19.3 ± 1.5 | 43.6 ± 7.0* | 22.0 ± 3.4# | |||
| Creatinine | 0.18 ± 0.01 | 0.20 ± 0.01 | 0.36 ± 0.03* | 0.24 ± 0.03# | |||
| Plasma NOx | 15.2 ± 4.4 | 19.3 ± 4.8 | 138.8 ± 15.6* | 99.2 ± 4.6* | |||
| Plasma cGMP | 12.0 ± 0.2 | 12.3 ± 1.0 | 18.7 ± 0.3* | 8.8 ± 0.7*# | |||