LPS-induced pulmonary neutrophilia
LPS was instilled into the nares of BALB/c mice, followed 30 min later by treatment with aerosolized vehicle (water/tyloxapol), aerosolized TG100-115, or systemic steroid. Four hours after LPS administration, BALF was collected and analyzed for neutrophil counts (presented as percentage of total cells present) and TNFα levels. Data are presented as means ± S.E.M. Study 1, n = 4, all groups differ from one another by P < 0.01 for both measures. Study 2, n = 5 to 6, all groups differ from one another by P < 0.01 for both measures except for the 0.04 and 0.4% TG100-115 groups and the 0.004% TG100-115 and non-LPS control groups.
Study | LPS | Treatment | Neutrophils | TNFα |
|---|---|---|---|---|
| % Total Cells | pg/ml | |||
| Study 1 | No | Aerosolized vehicle | 4 ± 3 | 22 ± 7 |
| Yes | Aerosolized vehicle | 64 ± 6 | 1836 ± 178 | |
| Yes | Aerosolized TG100-115 (0.4% formulation) | 39 ± 5 | 952 ± 182 | |
| Yes | Dexamethasone (0.3 mg/kg i.p.) | 22 ± 3 | 346 ± 68 | |
| Study 2 | No | Aerosolized vehicle | 16 ± 4 | 256 ± 59 |
| Yes | Aerosolized vehicle | 53 ± 4 | 1784 ± 240 | |
| Yes | Aerosolized TG100-115 (0.004% formulation) | 18 ± 5 | 738 ± 227 | |
| Yes | Aerosolized TG100-115 (0.04% formulation) | 36 ± 3 | 1077 ± 109 | |
|
| Yes | Aerosolized TG100-115 (0.4% formulation) | 33 ± 1 | 1267 ± 115 |