TABLE 2
Naloxone-precipitated withdrawal signs do not differ in morphine-dependent WT and GluR5 KO mice On day 4 after placebo (PLAC) or 25-mg morphine (MOR) pellet implantation, withdrawal was precipitated in WT or GluR5 KO mice (n = 8) by subcutaneous injection of naloxone (0.2 mg/kg) 3 h after removal of the pellet. We measured the number of jumps and wet dog shakes occurring within the first 15 min and the amount of weight loss in grams at 2 h after naloxone injection. In separate groups of WT and GluR5 KO mice, osmotic pumps delivering saline (SAL) or LY293558 at 60 mg/kg/24 h (LY) at 1 μl/h were implanted subcutaneously 16 h before insertion of the morphine pellet and then removed along with the pellet before naloxone injection.