Suppression of microtubule dynamic instability by carbendazim in living MCF7/GFP cells Values are expressed as mean ± S.E.M. Results are from 30 to 70 individual microtubules from 8 to 15 cells for each condition.
Control | Carbendazim, 5 μM | Change | Carbendazim, 15 μM | Change | Carbendazim, 30 μM | Change | |
|---|---|---|---|---|---|---|---|
| % | % | % | |||||
| Growth rate (μm/min) | 9.5 ± 0.4 | 9.0 ± 0.4 | -5 | 8.1 ± 0.3** | -15 | 7.0 ± 0.6*** | -26 |
| Growth length (μm) | 1.9 ± 0.1 | 1.4 ± 0.1** | -26 | 1.6 ± 0.1 | -16 | 0.8 ± 0.1*** | -58 |
| Shortening rate (μm/min) | 17.8 ± 1.1 | 15.8 ± 1.0 | -11 | 11.1 ± 0.7*** | -38 | 7.4 ± 0.7*** | -58 |
| Shortening length (μm) | 3.4 ± 0.3 | 2.3 ± 0.2*** | -32 | 1.9 ± 0.1*** | -44 | 1.0 ± 0.1*** | -71 |
| Catastrophe frequency (per min) | 2.5 ± 0.2 | 3.1 ± 0.3 | +24 | 2.3 ± 0.2 | -8 | 2.2 ± 0.3 | -12 |
| Rescue frequency (per min) | 7.3 ± 0.9 | 8.3 ± 0.6 | +14% | 5.9 ± 0.5 | -19 | 7.2 ± 1.0 | -1 |
| Dynamicity (μm/min) | 9.5 ± 0.8 | 8.9 ± 0.9 | -6 | 5.6 ± 0.5*** | -41 | 3.2 ± 0.6*** | -66 |
| Percentage of total time growing | 30.5 | 29.9 | 28.2 | 18.7 | |||
| Percentage of time shortening | 27.2 | 25.9 | 25.8 | 23.2 | |||
| Percentage of total time attenuated | 42.3 | 44.2 |
| 46.0 |
| 58.1 |
|