Agonist activity for stimulating phosphoinositide hydrolysis in HEK Gα15 M4 cells The data are from Fig. 4. The data represent the mean estimates ± S.E.M. The values in parentheses to the right of some of the estimates are the Log mean ± S.E.M.
Agonist | Emax a | EC50 | Hill Slope | RAi | ||
|---|---|---|---|---|---|---|
| Null | Operationalb | |||||
| % | μM | |||||
| Oxotremorine-M | 97 ± 1.1 | 0.078 (–7.11 ± 0.04) | 0.82 ± 0.06 | 8.01 (0.90 ± 0.04) | 10.67 (1.028 ± 0.12) | |
| Carbachol | 97 ± 0.9 | 0.90 (–6.05 ± 0.03) | 0.82 ± 0.03 | 1.0 (0.0) | 1.0 (0.0) | |
| McN-A-343 | 70 ± 1.4 | 1.1 (–5.98 ± 0.06) | 0.87 ± 0.08 | 0.65 (–0.19 ± 0.07) | 0.44 (–0.36 ± 0.05) | |
| S-Aceclidine | 91 ± 1.7 | 0.62 (–6.21 ± 0.05) | 0.82 ± 0.07 | 1.42 (0.15 ± 0.06) | 1.24 (0.09 ± 0.06) | |
| R-Aceclidine | 61 ± 1.9 | 2.7 (–5.56 ± 0.08) | 0.92 ± 0.13 | 0.22 (–0.65 ± 0.04) | 0.13 (–0.87 ± 0.08 | |
↵ a Denotes the maximum stimulation of phosphoinositide hydrolysis by carbachol
↵ b The operational RAi values for oxotremorine-M and carbachol were estimated using eqs. 10, 11, 12, 13, whereas those for McN-A-343, S-aceclidine, and R-aceclidine were estimated using eqs. 8 and 9. In each analysis the concentration-response curve of carbachol was analyzed simultaneously as the standard