Apparent kinetic parameters for sulfation of XA by recombinant rat and human STs Assays were performed as described in the legend to Table 1 with 5 ng of recombinant proteins. All values are the mean ± S.D. from three independent experiments (n = 2–3 in each experiment).
ST | Km | Vmax | Vmax/Km |
|---|---|---|---|
| μM | nmol/min/mg protein | ||
| rSult1b1 | 0.52 ± 0.07 | 1.05 ± 0.04 | 2.0 ± 0.02 |
| hSULT1B1 | 6.3 ± 0.3 | 14.5 ± 1.1 | 2.3 ± 0.03 |
| hSULT1A1 | 43.3 ± 2.7 | 51.1 ± 1.5 | 1.2 ± 0.02 |
| hSULT1C2 | 205.7 ± 16.6 | 57.2 ± 3.5 | 0.3 ± 0.00 |