TABLE 2

Affinity and density of D3 receptors after experimental treatments Pooled ventral striatal membranes (nucleus accumbens and olfactory tubercle) from mice in each treatment group were assayed for D3 dopamine receptor binding using [3H](+)-PD-128,907 (6–8 concentrations per assay, 0.02–3.2 nM). Data represent the mean of two independent determinations using LIGAND. The S.D. between determinations for Kd values ranged between 12 and 54% of the mean; Bmax values, between 5 and 16% of the mean.


Treatment

Kd

Bmax
nM fmol / mg protein
Saline-saline 0.65 18.6
Saline-cocaine 0.64 24.0
(+)-PD-128,907-saline 1.4 16.9
(+)-PD-128,907-cocaine 0.63 30.9*
Acute cocaine
0.48
22.0
  • * Effects significantly different from saline-saline treatment effects