TABLE 5

Comparison of protein levels and mRNA levels of Kcnb1 in mouse brain Western blot analysis of Kcnb1 protein was performed as described in the text. Densitometric measures were derived from images such as the ones illustrated in Fig. 4. mRNA levels were obtained through analysis of Affymetrix MOE430V2 arrays. Data are mean ± S.E.M. There were six mice/strain used in Western blot analysis and 4 to 6 mice/strain used in microarray analysis. BXD 13 mice display high AFT, whereas BXD 38 mice display low AFT. HAFT1/LAFT1 mice were selectively bred for high (HAFT) and low (LAFT) levels of AFT. A t test was used to compare expression values between BXD13 mice and BXD 38 mice and between HAFT1 mice and LAFT1 mice.




mRNA Microarray Analysis

Protein Western Blot Analysis

Microarray Analysis

Western Blot
BXD 13 9.19 ± 0.06**** 1.96 ± 0.13**
BXD 38 9.95 ± 0.05**** 2.52 ± 0.11**
HAFT1 8.39 ± 0.04*** 1.01 ± 0.07*
LAFT1


9.13 ± 0.13***
1.22 ± 0.07*
  • * p < 0.05

  • ** p < 0.01

  • *** p = 0.002

  • **** p = 0.00002