Mutations | Primer Sequence | RESa |
---|---|---|
C43A | TTTCATAACATTGCCTATCGAGTAAAACTGAAG | BsrDI |
C55A | GCTTTCTACCTGCACGAAAACCAGTTGAG | BsgI |
C119A | GCCAATTTCAAAGCGAATTCAGGTTACGTGG | EcoRI |
C284A | GAATCAGCTGGATATCACGCTGAGGCCTATAATAAC | EcoRV |
C374A | ACACCACCTCCTTCGCTCATCAACTCAGATG | None |
C438A | CTGACGACCAACCAAGCTTTCAGCAGTGTTTC | HindIII |
C491A | TATATTTACCGCTATAGTATACTTCATGTTAGG | AccI |
C544A | CTTCTCATGACGATCGCTTTTGTGTTTATGATG | PvuI |
C592A | GGACAAAACTTCGCCCCGGGACTCAATGCAA | SmaI |
C603A/C608A | AGGAAACAATCCTGCTAACTATGCAACAGCTACTGGCGAAGAATATTT | -NspI |
C635A | CACGTGGCCTTGGCTGCAATGATTGTTATTTTC | BsrDI |
↵ a Restriction (RES) enzyme digestion sites engineered in the primer for the convenience of detection. The primer sequence for the C603A/C608A mutant does not contain the NspI site present in the wild-type sequence.