EETs | Vmax | Km | |
---|---|---|---|
pmol/min/108 RBCs μM | |||
14,15-trans-EET | 3.59 ± 0.37 | 1.29 ± 0.35 | |
11,12-trans-EET | 2.72 ± 0.22 | 1.15 ± 0.25 | |
8,9-trans-EET | 1.24 ± 0.24 | 2.24 ± 0.89 | |
14,15-cis-EETa | 3.06 ± 0.90 | 0.91 ± 0.65 | |
11,12-cis-EET | 0.77 ± 0.10 | 0.69 ± 0.30 | |
8,9-cis-EET | 0.36 ± 0.06 | 1.55 ± 0.61 |
↵ a Values were obtained by analyzing substrate concentrations from 0.2 to 2 μM. Hydrolysis of 14,15-cis-EET at 4 μM by erythrocyte cytosol was greatly enhanced, not conforming to Michaelis-Menten kinetics possibly due to further hydrolysis by LTA4 hydrolase (McGee and Fitzpatrick, 1985).