Parent and metabolite compounds in plasma and brain after chronic dietary administration
Curcumin or TC in chow was fed to mice for 4-month duration at 500 ppm (∼2.5 mg/day) or 2000 ppm (∼10 mg/day). After sacrifice, plasma was collected, animals were perfused, and brains were removed. Plasma values were detected using HPLC, and the lower limits of detection for curcumin and TC were 35 and 8 ng/ml, respectively. Brain levels were measured using LC/MS/MS, and the lower limits of detection were 100 pg/g t for curcumin and 1 ng/g t for TC. Data represent mean ± S.D.
Dose Chow | PC | Plasma Curc | Plasma TC | Brain Curc | Brain TC | Brain-to-Plasma Ratio (PC) | ||
|---|---|---|---|---|---|---|---|---|
| μg/ml or (μM) | μg/g t or (μM) | |||||||
| 0 ppm | Con | N.D. | N.D. | N.D. | N.D. | N.A. | ||
| 500 ppm | Curc | 0.035 ± 0.014 (0.095 μM) | 0.008 ± 0.001 (0.023 μM) | 0.469 ± 0.220 (1.276 μM) | 0.097 ± 0.008 (0.264 μM) | 13.4 | ||
| 2000 ppm | Curc | 0.171 ± 0.019 (0.465 μM) | 0.042 ± 0.002 (0.115 μM) | 0.525 ± 0.125 (1.428 μM) | 0.052 ± 0.054 (0.143 μM) | 3.07 | ||
| 500 ppm | TC | N.D. | 0.270 ± 0.003 (0.734 μM) | N.D. | 0.128 ± 0.019 (0.344 μM) | 0.47 | ||
N.A., not applicable; N.D., not detectable; t; wet tissue.