Biodistribution of anti-ICAM/ASM vs. IgG/ASM nanocarriers in mice
Blood and organ levels of 180-nm-diameter polystyrene particles carrying 125I-ASM and either anti-ICAM or control IgG 30 min after i.v. injection in anesthetized C57Bl/6 mice. Data are mean ± S.E.M. (n ≥ 6 mice).
Blood | Kidney | Heart | Liver | Spleen | Lung | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Anti-ICAM | IgG | Anti-ICAM | IgG | Anti-ICAM | IgG | Anti-ICAM | IgG | Anti-ICAM | IgG | Anti-ICAM | IgG | |||||||
| %ID | 4.1 ± 0.4 | 3.4 ± 0.3 | 1.4 ± 0.05* | 0.9 ± 0.1 | 0.4 ± 0.03 | 0.3 ± 0.04 | 39.0 ± 2.8*** | 58.9 ± 1.9 | 4.0 ± 0.4 | 4.6 ± 0.9 | 22.8 ± 3.0*** | 0.9 ± 0.2 | ||||||
| %ID/g | 2.6 ± 0.3 | 2.0 ± 0.1 | 4.7 ± 0.2** | 3.2 ± 0.5 | 3.7 ± 0.3 | 3.1 ± 0.5 | 36.4 ± 2.8** | 53.9 ± 2.6 | 47.3 ± 5.1 | 58.2 ± 14.2 | 140.8 ± 17.0*** | 6.9 ± 1.5 | ||||||
| LR | 2.0 ± 0.2 | 1.3 ± 0.4 | 1.6 ± 0.2 | 1.1 ± 0.2 | 16.6 ± 2.5 | 20.0 ± 4.6 | 22.3 ± 3.8 | 20.7 ± 7.8 | 61.5 ± 11.3*** | 2.9 ± 1.1 | ||||||||
| Specificity index |
|
| 1.5 |
| 1.5 |
| 0.8 |
| 1.1 |
| 21.3 |
| ||||||
↵* p < 0.05, ** p < 0.01, and *** p < 0.001 (Student's t test).