Primer sequences
Target | Primer Sequences | Amplicon Size Efficiency (%) |
|---|---|---|
| bp | ||
| 18S | F: 5′-gaggttcgaagacgatcaga | 316 |
| R: 5′-tcgctccaccaactaagaac | 100.29 | |
| Calpain 8 | F: 5′-agaagatggcgagttctgga | 190 |
| R: 5′-ccagtacgtgcctgggtagt | 102.00 | |
| Calpain 2 | F: 5′-tgccccagctggaacacg | 149 |
| R: 5′-caggtgagggtgtccgg | 95.25 | |
| Calpain 1 | ||
| Set 1 | F: 5′-aggaggtggatgacgcagac | 141 |
| R: 5′-ggacctggtacactgcaaaac | 97.50 | |
| Set 2* | F*: 5′-tatcctccaccgagtggttc | 129 |
| R*: 5′-tccttggtgggtagcaaatc | 89.35 | |
| Calpastatin | F: 5′-tgcaggagtgaccagaagtg | |
| R: 5′-gcttgcctaagatggctctg | 144 | |
|
| P: 5′-56-FAM-aagccctgcaggctctgtcagattc-TAM | 102.72 |