Effects of BODIPY-FS on the activity of AC2 expressed with various virus titers
Sf9 membranes were infected with AC2 virus at the titers given in the table. Membranes were prepared after a 48- h infection. AC activity was determined as described under Materials and Methods. Each assay tube contained a specific membrane (30 μg protein/tube), [α-32P]ATP (1.0-1.5 μCi/tube), 40 μM unlabeled ATP, Mn2+ (10 mM) and GTPγS (10 μM), 3% (v/v) DMSO, and increasing concentrations (10 nM-30 μM) of BODIPY-FS. Data were analyzed by nonlinear regression and best fitted to sigmoidal concentration/response curves. Data shown are mean values ± S.D. of three independent experiments in duplicate.
Virus Titer | AC Activity (Basal) | AC Activity (BODIPY-FS) | Relative Inhibitory Effect of BODIPY-FS | EC50 | |
|---|---|---|---|---|---|
| pmol/mg/min | % | mM | |||
| 1:10 | 44.1 ± 2.6 | 27.7 ± 0.9 | -37.2 ± 1.7 | 1.1 ± 0.5 | |
| 1:100 | 40.1 ± 3.9 | 23.1 ± 2.1 | -42.4 ± 2.9 | 0.6 ± 0.4 | |
| 1:300 | 32.5 ± 3.1 | 20.7 ± 1.3 | -36.3 ± 1.9 | 0.5 ± 0.2 | |