Kinetic values for transport of [14C]α-methyl-d-glucopyranoside in wild-type and mutant SGLT transporters expressed in COS-7 cells
The data shown are means ± S.E.M. followed by the number of separate experiments.
Parental | Mutation | Km | Vmax | n |
|---|---|---|---|---|
| mM | pmol / well / min | |||
| hSGLT1 | wt | 1.8 ± 0.4 | 1010 ± 213 | 4 |
| Y606L | 1.8 ± 0.5 | 559 ± 245 | 3 | |
| Y606K | 3.3 ± 1.1 | 536 ± 110 | 3 | |
| C610K | 3.0 ± 0.9 | 372 ± 93 | 4 | |
| hSGLT2 | wt | 4.8 ± 1.7 | 143 ± 30 | 3 |
| L611Y | 2.0 ± 1.1 | 218 ± 65 | 3 | |
| L611K | 2.5 ± 0.1 | 220 ± 7 | 3 | |
| C615K | 4.5 ± 1.2 | 222 ± 31 | 5 | |
| mSGLT2 | wt | 6.0 ± 1.5 | 643 ± 105 | 3 |
|
| N173A | 17.9 | 547 | 1 |
wt, wild type.