TABLE 1

Dectin-1 binding constants (IC50) for natural product and synthetic β-d-glucans


Glucan

Type of Glycosidic Linkage

Mr

IC50a

CIs (95%)
g/mol
Heptasaccharideb β-(1→3) 1.15 × 103 >1 mM
Branched heptasaccharidec β-(1→3), (1→6) 1.33 × 103 0.13 mM 0.11-0.15 mM
Linear octasaccharidec β-(1→3) 1.33 × 103 1.1 mM 1.8-4.3 mM
Branched octasaccharidec β-(1→3), (1→6) 1.51 × 103 1.3 mM 1.1-1.5 mM
Linear nonasaccharidec β-(1→3) 1.51 × 103 2.6 mM 2.0-3.4 mM
Branched nonasaccharidec β-(1→3), (1→6) 1.69 × 103 29 μM 20-41 μM
Linear decasaccharidec β-(1→3) 1.69 × 103 0.7 mM 0.57-0.79 mM
Laminarind β-(1→3), (1→6) 7.70 × 103 22 nM 17-29 nM
C. albicans hyphal glucane β-(1→3), (1→6) 3.3 × 104 440 pM 350-560 pM
C. albicans blastospore glucane β-(1→3), (1→6) 1.0 × 105 3.4 nM 2.9-4.1 nM
Glucan phosphatef β-(1→3) 1.55 × 105 2.2 pM 1.7-2.7 pM
Scleroglucang
β-(1→3), (1→6)
1.02 × 106
102 pM
83-25 pM
  • a IC50 is the carbohydrate concentration required to inhibit 50% of Dectin-1 binding to a glucan-coated biosensor surface (Kougias, et al., 2001).

  • b Derived from a natural product source as described by Whelan (1963).

  • c Synthetic glucan oligosaccharides were prepared as described by Yu et al. (2005).

  • d Obtained from Sigma-Aldrich and described by Kim et al. (2000).

  • e As described by Lowman et al. (2003).

  • f As described by Williams et al. (1991), Ensley et al. (1994), and Mueller et al. (2000).

  • g As described by Pretus et al. (1991).