TABLE 3

Alfuzosin stimulation of late sodium current

Unitary currents were recorded from HEK293 cells stably expressing hNa_v1.5 with the cell-attached configuration of the patch-clamp method. Patches contained multiple sodium channels, and openings were evoked with the voltage protocol described in the legend to Fig. 4. NP_o for late openings was measured over the last 65 ms of a 125-ms step to -40 mV. Alfuzosin was applied to the bath at 300 nM and 10 μM concentrations and increased late openings. The mean increase at 300 nM was 52 ± 10% (n = 3) and was statistically significant (p < 0.05).