Effect of dexamethasone and/or calcitriol on the inflammatory response of acute TNBS colitis
TNBS-treated mice were treated i.p. with calcitriol and/or dexamethasone. IFNγ, TNFα, MPO activity, and IL-1β were determined on day 3. The results are the mean ± S.E.M. from three separate experiments (8 mice/group/experiment).
TNFα | IFNγ | MPO Activity | IL-1β | ||
|---|---|---|---|---|---|
| pg / mg protein | U / g colon tissue | pg / mg protein | |||
| Control | 5.46 ± 0.42 | 5.91 ± 0.58 | 0.17 ± 0.09 | 4.23 ± 0.39 | |
| TNBS | 94.38 ± 9.55 | 84.23 ± 7.94 | 10.91 ± 0.90 | 112.70 ± 14.99 | |
| Calcitriol (0.2 μg/kg) | 45.88 ± 3.72b | 34.72 ± 9.82b | 7.83 ± 0.18c | 39.29 ± 5.07c | |
| Dex (0.6 mg/kg) | 34.44 ± 8.67b | 26.67 ± 5.09c | 4.29 ± 0.88c | 32.18 ± 5.54c | |
| Dex (0.6 mg/kg) + calcitriol (0.2 μg/kg) | 22.99 ± 3.73c | 18.54 ± 4.35c | 1.77 ± 0.68c | 24.47 ± 5.91c | |
| Dex (0.9 mg/kg) | 29.47 ± 2.62c | 16.21 ± 3.94c | 2.25 ± 0.72c | 19.39 ± 7.88c | |
| Dex (0.9 mg/kg) + calcitriol (0.2 μg/kg) | 16.77 ± 3.74c | 11.09 ± 4.24c | 1.23 ± 0.78c | 11.89 ± 4.50c | |
| Dex (1.2 mg/kg) | 11.65 ± 0.76c | 11.09 ± 2.55c | 1.29 ± 0.72c | 10.77 ± 1.29c | |
| Dex (1.2 mg/kg) + calcitriol (0.2 μg/kg) | 8.13 ± 1.22c | 7.49 ± 2.11c | 0.82 ± 0.23c | 6.36 ± 0.48c | |