Primer sequences used in this study
Gene | Primer Sequences for RT-PCR | Size |
|---|---|---|
| base pairs | ||
| EGR-1 | ||
| F | 5′-CAGTGGCCTAGTGAGCATGA-3′ | 787 |
| R | 5′-CCGCAAGTGGATCTTGGTAT-3′ | |
| NAG-1 | ||
| F | 5′-ACTCCGAAGACTCCAGATTCCGA-3′ | 583 |
| R | 5′-ATGCACATGGTCACTTGCACCT-3′ | |
| NRG-1 | ||
| F | 5′-CCATCGTGGAATCAAACGAGATC-3′ | 551 |
| R | 5′-TGATTCACCAGCTGGACATTCTC-3′ | |
| FGF-2 | ||
| F | 5′-GCTCAAGCTGGAGTGGGTCTA-3′ | 98 |
| R | 5′-ATAGACCCACTCCAGCTTGAGC-3′ | |
| FEM-1 | ||
| F | 5′-TACGCTTGCTCTTAGAACATTACC-3′ | 811 |
| R | 5′-CGAACTATAAGGCCTTCCATATG-3′ | |
| Cullin5 | ||
| F | 5′-GAAGCAAAAAGGAGTGGGGTT-3′ | 700 |
| R | 5′-CTAAAACTTACGACCACGAACC-3′ | |
| GAPDH | ||
| F | 5′-TCAACGGATTTGGTCGTATT-3′ | 467 |
| R | 5′-CTGTGGTCATGAGTCCTTCC-3′ |
|
F, forward; R, reverse.