Effects of berberine on mitochondrial respiratory parameters energized by succinate
Data represent percentage against control. Experiences were carried out in the same conditions as describe in Table 3 but with mitochondria energized with 5 mM succinate in the presence of 3 μM rotenone. Data are means ± S.E.M. of four to five different preparations. Control absolute values are expressed as nanoatoms of oxygen per minute per milligram of protein. Other values are expressed as percent control values.
Berberine | Control | 6.7 nmol/mg Protein | 16.7 nmol/mg Protein | 33.4 nmol/mg Protein | 66.7 nmol/mg Protein | |||
|---|---|---|---|---|---|---|---|---|
| % | ||||||||
| State 3 | 90.7 ± 10.2 | 90.8 ± 5.6 | 75.8 ± 1.8* | 74.6 ± 3.6* | 58.6 ± 4.7* | |||
| State 4 | 22.6 ± 1.6 | 96.4 ± 5.7 | 110.8 ± 8.1 | 127.7 ± 3.2* | 143.0 ± 8.8* | |||
| RCR | 4.1 ± 0.4 | 93.8 ± 4.1 | 69.1 ± 4.4* | 62.4 ± 7.0* | 44.9 ± 4.9* | |||
| ADP/O | 1.6 ± 0.2 | 91.8 ± 3.0 | 94.1 ± 3.0 | 83.5 ± 1.3* | 81.4 ± 3.2* | |||
| Oligomycin | 16.4 ± 2.1 | 94.5 ± 3.5 | 105.9 ± 4.7 | 113.8 ± 6.2 | 108.9 ± 9.4 | |||
| FCCP | 152.7 ± 16.2 | 85.8 ± 4.1 | 79.7 ± 3.9* | 70.6 ± 7.2* | 52.0 ± 4.4* | |||
↵* p < 0.05 vs. control.