TABLE 1

Expression and activity data for paired tumor (T) and normal (N) colon tissue samples

Ten microliters of colon tissue lysate was incubated with 0.5 mM 4-MUA in 90 mM potassium phosphate containing 40 mM potassium chloride buffer, pH 7.4, at 37°C. Specific activity for 4-MUA was calculated in micromoles per milligram per hour. CES2 band density was determined by running 50 to 100 μg of colon tissues lysates on nondenaturing PAGE. The gels were exposed to 1 mM 4-MUA in 100 mM potassium phosphate buffer, pH 6.0, for 10 min at room temperature. Band density was measured by fluorescence. Band densities were normalized to CES2 controls to account for variations between gels.