Name, sequence of forward and reverse primer, size of the PCR product, extension time, and melting temperature for each gene
Gene | Primer Name | Primer Sequences (5′-3′) | Size | Extension Time | Melting Temp. |
|---|---|---|---|---|---|
| pb | s | °C | |||
| rPepT1 | rPepT1-F | TGTTCTGTTCGCCTCCTTG | 224 | 9 | 63 |
| rPepT1-R | TGGTGAATGCTGGACTTGG | ||||
| rβ-Actin | rβ Actin-F | CCAGCCATGTACGTAGCCAT-C | 103 | 5 | 60 |
| rβ Actin-R | ACAGTGTGGGTGACCCCGT | ||||
| rGAPDH | rGAPDH-F | GTTACCAGGGCTGCCTTCTC | 168 | 7 | 64 |
| rGAPDH-R | GGGTTTCCCGTTGATGACC | ||||
| rUbiquitin C | rUbiquitin C-F | TCGTACCTTTCTCACCACAGTATCTAG | 83 | 4 | 60 |
| rUbiquitin C-R | GAAAACTAAGACACCTCCCCATCA | ||||
| mPepT1 | mPepT1-F | CGTGCACGTAGCACTGTCCAT | 66 | 5 | 60 |
| mPepT1-R | GGCTTGATTCCTCCTGTACCA | ||||
| mβ-Actin | mβ Actin-F | AGAGGGAAATCGTGCGTGAC | 139 | 6 | 60 |
| mβ Actin-R | CAATAGTGATGACCTGGCCGT | ||||
| mGAPDH | mGAPDH-F | TGCACCACCAACTGCTTAG | 177 | 7 | 60 |
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| mGAPDH-R | GATGCAGGGATGATGTTC |
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m, mouse; r, rat; -F, forward; -R, reverse.